Raw and analyzed data for manuscript "In vitro eradication of Candida albicans biofilm through cold atmospheric plasma: Unravelling the interdependence of exposure and voltage in the antifungal mode of action."

Raw and analysed data for the manuscript, to be submitted to Journal of Infection and Public Health.

Abstract:

Introduction: Since Candida spp. is the fourth leading cause of healthcare-associated infections globally, the need for novel antifungal agents is increasing among scientists. This study investigates the potential of cold atmospheric plasma for C. albicans biofilm treatment. Methods: Our research focused on in vitro C. albicans biofilm response to varying parameters of plasma application, specifically the impact of treatment duration and input voltage. Evaluation of plasma influence on C. albicans was assessed with viability, membrane integrity, and oxidative stress measurements, along with observations of biofilm chemical composition and hyphae growth after plasma treatment. Results and Discussion: The higher plasma input voltage and increased exposure tme resulted in lower C. albicans cell viability, with complete reductions observed after 5 min plasma treatment duration across all input voltages tested. The effect of plasma treatment was further confirmed with a microscopic examination after BacLight® staining. Low (8 V) CAP exposure could potentially lead to a phenomenon known as hormesis, which was observed in C. albicans 24 h growth measurements. Additionally, intracellular oxidative stress assessment further proved that with prolonged treatment times, the intensity of oxidative stress, increased. Plasma treatment also affected the hyphae, which exhibited signs of contraction and compression. The chemical characteristics revealed that increasing plasma voltage and exposure time also have a distinguished impact on lipids, proteins, and carbohydrates, typical constituents of fungi biofilms. Conclusion: This study underscores the potential of plasma as a promising approach in combating Candida spp. biofilms, shedding light on the intricate dynamics of its impact on biofilm viability, morphology and composition.