Contrasting roles of the RSC and ISW1/CHD1 chromatin remodelers in RNA polymerase II elongation and termination

We addressed the roles of four remodeling machines (ISW1, ISW2, CHD1 and RSC) in specifying the chromatin organization. Overall design: Comparison of budding yeast nucleosome positions obtained using micrococcal nuclease digestion followed by paired-end sequencing (MNase-seq). We sequenced nucleosomes from isogenic strains with the essential RSC8 gene linked to a GAL promoter and isw1?, isw2? or chd1? mutations in all combinations. We measured gene activity by ChIP-seq for the Rpb3 subunit of RNA polymerase II.