Transcriptomic profiling of circadian genes in WT mouse tissues (miRNA-Seq)

A goal of a collaboration between the CHDI Foundation and Prof. Jenny Morton at Cambridge University was to identify genes in mice with diurnal/circadian expression patterns. Tissues from wild type mice were harvested every 3 hrs over a 36 hour time period, for a total of 13 time points. Striatal, cortical, hypothalamic and liver samples were harvested, flash frozen, and transferred to Expression Analysis for RNA extraction and miRNA-seq sequencing. Samples passing QC were analyzed to produce high quality, 50-base paired-end RNA-seq results. Striatal, cortical, hypothalamic and liver samples were collected from 26-week-old wild type C57BL/6J male mice every 3 hours for 36 hours, number of replicates 2 to 12 for each ZT group. miRNA-Seq was performed.CHDI基金会与剑桥大学珍妮·莫顿教授开展合作的核心目标之一，是鉴定具备昼夜（diurnal/circadian）表达特征的小鼠基因。研究团队在36小时的周期内，每3小时采集一次野生型小鼠的组织样本，共计13个时间点。研究人员采集了纹状体、皮层、下丘脑及肝脏样本，经快速冷冻后送至Expression Analysis公司完成RNA提取与miRNA-seq测序。通过质量控制（QC）的合格样本将用于后续分析，以生成高质量的50碱基双端RNA-seq测序结果。本次研究的样本取自26周龄的野生型C57BL/6J雄性小鼠，以每3小时一次的频率采集，持续时长为36小时；每个授时时间（ZT）组的生物学重复数为2至12份。目前已完成miRNA-seq测序。