Table_2_Genome-Wide Identification of MATE Gene Family in Potato (Solanum tuberosum L.) and Expression Analysis in Heavy Metal Stress.XLSX

A genome-wide identification and expression analysis of multidrug and toxic compound extrusion (MATE) gene family in potato was carried out to explore the response of MATE proteins to heavy meta stress. In this study, we identified 64 MATE genes from potato genome, which are located on 12 chromosomes, and are divided into I–IV subfamilies based on phylogenetic analysis. According to their order of appearance on the chromosomes, they were named from StMATE1–64. Subcellular location prediction showed that 98% of them are located on the plasma membrane as transporters. Synteny analysis showed that five pairs of collinearity gene pairs belonged to members of subfamily I and subfamily II had two pairs indicating that the duplication is of great significance to the evolution of genes in subfamilies I and II. Gene exon–intron structures and motif composition are more similar in the same subfamily. Every StMATE gene contained at least one cis-acting element associated with regulation of hormone transport. The relative expression levels of eight StMATE genes were significantly upregulated under Cu2+ stress compared with the non-stress condition (0 h). After Cd2+ stress for 24 h, the expression levels of StMATE33 in leaf tissue were significantly increased, indicating its crucial role in the process of Cd2+ stress. Additionally, StMATE18/60/40/33/5 were significantly induced by Cu2+ stress, while StMATE59 (II) was significantly induced by Ni2+ stress. Our study initially explores the biological functions of StMATE genes in the regulation of heavy metal stress, further providing a theoretical basis for studying the subsequent molecular mechanisms in detail.

本研究对马铃薯中多药和有毒化合物外排（MATE）基因家族进行了全基因组鉴定和表达分析，旨在探讨MATE蛋白对重金属胁迫的响应。通过对马铃薯基因组的研究，我们共鉴定出64个MATE基因，这些基因分布在12条染色体上，根据系统发育分析被划分为I至IV个子家族。依据它们在染色体上的出现顺序，它们被命名为StMATE1至64。亚细胞定位预测表明，其中98%位于质膜上作为转运蛋白。同源分析显示，五对同线性基因对属于I和II个子家族的成员，而II个子家族有两个基因对，表明这种基因复制对于I和II个子家族基因的进化具有重要意义。基因外显子-内含子结构和基序组成在同一个子家族中更为相似。每个StMATE基因至少含有一个与激素运输调控相关的顺式作用元件。与无胁迫条件（0小时）相比，在Cu2+胁迫下，八种StMATE基因的相对表达水平显著上调。在Cd2+胁迫24小时后，叶片组织中StMATE33的表达水平显著升高，表明其在Cd2+胁迫过程中的关键作用。此外，StMATE18、60、40、33和5在Cu2+胁迫下显著诱导，而StMATE59（II）在Ni2+胁迫下显著诱导。本研究初步探讨了StMATE基因在调节重金属胁迫中的生物学功能，为进一步详细研究后续分子机制提供了理论依据。