five

Supplemental Material for Hawley et al., 2025

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Figshare2025-03-03 更新2026-04-28 收录
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https://figshare.com/articles/dataset/Supplemental_Material_for_Hawley_i_et_al_i_2025/28506611
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DeepVariant vcf files containing mutations against the D. mauritiana reference genome generated using the below methods: After sequencing, raw fastq files were adapter trimmed using Trim Galore (v0.6.10) and aligned to the D. mauritiana reference genome (​​D_mauritiana_ASM438214v1; RefSeq) using bwa mem (v0.7.17). Aligned reads were then deduplicated by processing them through samtools (v1.18) fixmate, sort, and markdup with the -r argument. Variant calling was run on these deduplicated reads using deepvariant (v1.5.0) with the Whole Genome Sequence (WGS) model type argument
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2025-03-03
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