RNAseq analysis of human CD4+ regulatory Tregs and Tconvs in COVID-19 and healthy donors isolated from peripheral blood.
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE179448
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RNAseq analysis of human CD4+ regulatory Tregs and Tconvs in COVID-19 and healthy donors isolated from peripheral blood. Human Tregs and Tconvs from COVID-19 patients and healthy donors were magnetically isolated from frozen PBMCs using a Stem Cell kit, as CD4+CD25hiCD127lo and CD4+CD25–, respectively. Around 1,000 isolated cells were directly into lysis buffer for RNA-seq (Smart-seq2) library preparation following the standard ImmGen low-input protocol (www.immgen.org). Samples were collected and sequenced in two cohorts.
创建时间：
2021-10-06
