Wide-Field Calcium Imaging During Social Interaction

This zipped file contains one dataset collected during behavioral experiments in which mice explored a rectangular chamber measuring 27 × 15 cm. On the right side of the arena, there is a 1 cm wide gap that leads to an adjacent chamber of the same size (27 × 15 cm), which contains an unfamiliar mouse. Raw fluorescence image stacks were recorded from three mice (S09, S10, and S11), with each recording saved in NumPy binary format (.npy). The images have been spatially registered, with the anatomical landmarks bregma and lambda located at coordinates (100, 75) and (100, 145), respectively. To enhance signal clarity, pixels outside regions of neural activity have been masked and assigned a gray value of 0. Each pixel represents an area of 60 microns, and the image sequences were captured at a frame rate of 25 Hz. This dataset is intended for studies involving neural imaging, signal processing, and behavioral analysis during social exploration tasks. A CSV file is also included, containing the tracked coordinates of the animal’s centroid throughout the experiment. The x and y columns indicate the centroid’s position, while the fluo_frame column maps each point to the corresponding frame in the fluorescence imaging data. The time column provides timestamps in seconds, with zero marking the onset of blue light illumination used for GCaMP signal excitation. Pixel resolution is 0.44 mm.