Summary of the design and cutting efficiency of TALEN pairs.
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TALENs were assembled according to the protocol described by Cermak et al[1]. The target sequences of each TALEN pairs (TALEN-A and -B) are listed. Cutting efficiency for each TALEN pair was measured using the Surveyor endonuclease. Percentage NHEJ is indicated. The pairs used for gene editing in this study are indicated with an “X” in the last column. TAT: tyrosine aminotransferase; Sf3b1: Splicing factor 3B subunit 1
创建时间:
2015-12-02



