Cancer-associated SMARCAL1 loss-of-function mutations promote alternative lengthening of telomeres and tumorigenesis in telomerase-negative glioblastoma cells

In this study, we used a patient-derived ALT-positive GBM cell line with native SMARCAL1 deficiency to investigate the role of SMARCAL1 in ALT-mediated telomere synthesis and gliomagenesis in vitro and in vivo. Differential gene expression programs associated with tumorigenesis in D06MG xenografts. Comparative gene expression profiling analysis of RNA-seq data for Patient derived cells, subcutaneous tumor cells and the initial NSC medium-maintained cells