HUWE1 enhances WNT signaling through mechanisms mediated by a subset of destruction complex components including APC, AXIN1 and GSK3A or GSK3B.

Summary of effects of CRISPRi-mediated HUWE1 KD on the aggregate expression of four endogenous WNT target genes (Fig 4E; see figure legend for more details). For the genotypes and treatments indicated in green, both HUWE1 sgRNAs used resulted in a significant reduction in aggregate WNT target gene expression relative to the SCR sgRNA control.