Alarmin S100A11 initiates a chemokine response to the human pathogen Toxoplasma gondii

Purpose: The goal of this study is to compare NGS-derived transcriptome profiling of human PBMC infected with T. gondii, PRU and RH strains, (RNA-seq) to not stimulated human PBMC transcriptome (RNA-seq). Methods: After 24h post infection PBMC and non-infected PBMC mRNA profiles were generated by deep sequencing. The sequence reads were analyzed within Galaxy platform: FastQC(*) program was used to generate quality control reports followed by employment of Trimmamatic (*) to trim “noisy” ends; TopHat mapped reads to hg38 human genome; Cuffdiff (*) was used to find significant changes in transcript expression between infected with T. gondii human PBMC and not infected PBMC. Results: RNA-seq data for genes with a fold change =2.5 and p value <0.05. Conclusions: human innate responses to T. gondii were primarily characterized by the induction of chemokine expression, including CCL2 Overall design: Human PBMC mRNA profiles of infected cell with T. gondii and non infected cells were generated by deep sequencing,in quintuplicate