Cannabis sativa Genome sequencing and assembly

We describe the use of single molecule sequencing, assembly and annotation of a cannabis sativa breeding experiment. We shotgun sequenced and assembled a sibling pair and their offspring utilizing the Pacific Bioscience Sequel II platform. This resulted in the most contiguous cannabis sativa assemblies to date. These assemblies were further annotated with full-length mRNA sequencing (Iso-Seq) to help inform isoform complexity and gene model predictions and identify the Y chromosome. https://www.biorxiv.org/content/10.1101/2020.01.03.894428v1