A novel 2-bp indel within Krüppel-like factor 15 gene (<i>KLF15</i>) and its associations with chicken growth and carcass traits
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1. The Krüppel-like factor (KLF) family of zinc-finger transcription factors plays a critical role in cell differentiation, phenotypic modulation and physiologic function. <i>KLF15</i> has been proposed to regulate adipogenesis and gluconeogenesis. The objective of this study was to establish the association between <i>KLF15</i> gene polymorphism and chicken growth and carcass traits.2. An F<sub>2</sub>resource population of Gushi chickens crossed with Anka broilers was used to investigate the genetic effects of the chicken <i>KLF15</i> gene. A 2-bp indel mutation (G13781_13782del/insAG) within intron 2 was detected, and a polymerase chain reaction–restriction fragment length polymorphism method was developed to genotype the F<sub>2</sub> individuals.3. Association analysis showed that the Single Nucleotide Polymorphisms (SNP) was significantly associated with chicken growth and carcass traits. The chickens with the insAG/insAG genotype generally had a significantly higher body weight and size than other genotypes. Gene expression for each genotype showed that birds carrying insAG/insAG had a higher expression level than the other genotypes.4. The results suggested that this polymorphic site may serve as a useful target for marker assisted selection of chicken growth and carcass traits.
