Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides, and H2S in biological samples

This work describes the development and validation of a high-sensitivity analytical method (LC-MS/MS IAM-TPP) for the simultaneous quantification of protein persulfides, low molecular weight persulfides/polysulfides and hydrogen sulfide (H₂S) in complex biological matrices. The method was applied to biological samples to characterise H2S and endogenous persulfide/polysulfide formation, including analysis or physiologically relevant ischemia reperfusion models. The raw dataset is intended to enable independent re-analysis, method reuse, and further translational development in redox biology and drug discovery.
, The data derive from experiments described in the manuscript: using the IAM-TPP technique, persulfide and polysulfide species were trapped, derivatised, separated by liquid chromatography, and quantified by tandem mass spectrometry. Sample preparation, calibration curves, and validation experiments (linearity, limit-of-detection, reproducibility) are detailed in the publication.
Limitations: The dataset reflects specific sample types, instruments, and experimental conditions; normalisation and instrument-specific settings may differ in other laboratories. Raw data are provided to enable inspection of processing pipelines.
, # Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides, and H2S in biological samples

Dataset DOI: [10.5061/dryad.7m0cfxq8k](https://doi.org/10.5061/dryad.7m0cfxq8k)

## Description of the data and file structure

This dataset supports the publication: “Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides and H₂S in biological samples”.

### Files and variables

#### File: RAW_DATA.zip

**Description:** **Files and variables**

File: RAW_DATA.zip

**Description: File structure and contents**

RawData: Original instrument output files (LC-MS/MS raw data) corresponding to experiments presented in Figures 2, 4, 6, 5 and in Supplementary Figures 6, 7 and 8 of the main manuscript.  

\_extern.info: contain metadata describing each sample, date, instrument run ID, biological replicate.

## Code/software

**Code/software**

No open-source or publicly available software was used for data gener...,