Sampling details for non-target organisms used in specificity tests of the real-time PCR assay.

Note: a All the samples were identified with morphological first and then confirmed by DNA sequences. Asterisk (*) denotes samples which could not be identified morphologically and were identified using COI and/or ITS sequences, b DNA extractions were performed using several methods, QG = Qiagen DNeasy Blood and Tissue kit; QA = QIAamp DNA micro kit (Qiagen, CA, USA); ZR = ZR Tissue & Insect DNA kit (Zymo Research, CA, USA); PG = PrepGEM (ZyGem, USA). All the samples were tested negative to T. urticae in the real-time PCR assay in duplicate wells.