A pancreatic cancer cells spectral library and the phenotype analysis of gemcitabine-sensitive and -resistant pancreatic cell lines by the data-independent acquisition proteome strategy

Based on data-independent acquisition (DIA)-mass spectrometry (MS) strategies for in-depth proteome analysis, we established a high-coverage spectrum library from seven human pancreatic cell lines and evaluated performance of the library by exploring the molecular change between gemcitabine (GEM) sensitivity and resistance phenotypes .