The effect of mismatches and wobble base pairs in the upper stem of pri-miRNAs on DROSHA cleavage using small RNA sequencing
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE142136
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The experiments were conducted to check whether mismatches and wobble base pairs in the upper stem of pri-miRNAs affects the DROSHA cleavage and thereby miRNA biogenesis. HCT116 cells were transfected with pCDNA3 empty, pCDNA3 wild-type or mutant pri-miRNA plasmids. Small RNA sequencing libraries of the transfected HCT116 cells were generated using the TruSeq Small RNA Library Prep kit.
创建时间：
2020-05-07
