Transcriptomic analyses of ChREBPtotal-null mice and ChREBP-null mice subcutaneous inguinal white adipose tissue

The Carbohydrate Responive Element Binding Protein (ChREBP) is a transcription factor sensing glucose. Two isoforms are produced by the same gene, the long isoform ChREBPalpha and the short one ChREBPbeta transcribed from an alternative promoter. ChREBPalpha activates transcription of ChREBPbeta in response to glucose. The latter is thought to mediate most of the ChREBP transcriptional activation of target genes. However, the contribution of ChREBPbeta in metabolic adaptations in vivo is not known. This study aims at dertermining the contribution of the short isoform ChREBPbeta to the changes in gene expression mediated by ChREBP. The study contains 4 groups of mice: total ChREBP knock-out mice, ChREBPbeta knock-out mice, and the respective control wild type littermates of the two transgenic lines.碳水化合物反应元件结合蛋白（Carbohydrate Responsive Element Binding Protein，ChREBP）是一类感知葡萄糖的转录因子。同一基因可通过可变启动子转录产生两种同工型：长型同工型ChREBPα与短型同工型ChREBPβ。ChREBPα可响应葡萄糖信号激活ChREBPβ的转录，而后者被认为介导了ChREBP对靶基因的绝大多数转录激活事件。然而，体内代谢适应过程中ChREBPβ的具体贡献尚未明确。本研究旨在明确短型同工型ChREBPβ在ChREBP介导的基因表达变化中的作用。本研究共设置四组小鼠：全ChREBP敲除小鼠、ChREBPβ敲除小鼠，以及上述两种转基因品系对应的野生型同窝对照小鼠。