The GPR30 agonist G-1 promotes hair growth via Wnt/Hedgehog signaling in mice

Background: GPR30 is a membrane-associated receptor involved in rapid, non-genomic estrogen signaling. Estrogen significantly influences hair growth and susceptibility to hair loss, with differences primarily driven by hormonal factors. While estrogen’s role in regulating hair follicle cycling is recognized, its precise molecular mechanisms remain unclear. This study investigates the role of GPR30 in hair follicle biology and evaluates its potential as a therapeutic target for estrogen-mediated hair loss disorders.
Methods: The GPR30 selective agonist G-1 was administrated to female Gpr30-deficient mice with a C57BL/6J background and human hair follicle dermal papilla cell, and the effects on hair growth and the molecular signaling were evaluated.
Results: We demonstrate that GPR30 is abundantly expressed in mouse skin, particularly during the anagen phase of the hair follicle cycle, implicating it in hair growth regulation. Activation of GPR30 using the selective agonist G-1 in mouse s..., , , # The GPR30 agonist G-1 promotes hair growth via Wnt/Hedgehog signaling in mice

[https://doi.org/10.5061/dryad.xwdbrv1qj](https://doi.org/10.5061/dryad.xwdbrv1qj)

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#### File: Figure\_1.xlsx

*GPR30* was abundantly expressed in the skin.

**(A)** *Gpr30* mRNA expression in 7-week-old mouse tissues was determined using qRT-PCR (*n* = 3). Sub. Gland, submandibular gland; s. i., small intestine; bone.m, bone marrow; epi, epididymal; peri, perirenal; sub, subcutaneous; WAT, white adipose tissues; BAT, brown adipose tissues. **(B)** *Gpr30* mRNA expression in female mice during hair follicle cyclic phases was determined using qRT-PCR (*n* = 4). **(C)** *Gpr30* mRNA expression in male mice during hair follicle cyclic phases was determined using qRT-PCR (*n* = 4). Internal control: *18S* rRNA expression. ***p* < 0.01 (Tukey’s multiple comparison test). Results are presented as the means ± SE. N.S., not significant.

#### File: Figure\_2.xlsx

GPR30 selective agonist G-...,