HL-1 cardiac cells-derived extracellular vesicles containing microRNAs involved in myocardiogenesis and angiogenesis processes

HL-1 is an immortal cell line that shows a phenotype similar to adult cardiomyocytes. All major cardiac cell types release extracellular vesicles (EVs) that emerged as key mediators of intercellular communication. EVs can mediate intercellular cross-talk through the transfer of specific microRNAs (miRNAs). MiRNAs are known to play important regulatory roles during tissue differentiation and regeneration processes. Furthermore, miRNAs have recently been shown to be involved in the proliferation of adult cardiomyocytes. In this context, the purpose of this study was to analyze the transcriptomic profile of miRNAs expressed from EVs derived from HL-1 cardiac muscle cells, using Next-Generation Sequencing (NGS). Specifically, our transcriptomic analysis showed that the EVs derived from our HL-1 cells contain miRNAs, capable of promoting blood vessel formation and increasing cell proliferation. Indeed these miRNAs target several genes involved in biological processes related to myocardiogenesis and angiogenesis. Therefore, due to their content, EVs could acquire importance in the field of cardiovascular regenerative medicine.