Electron micrographs of parasites cultured with Shld-1.

Parasites were cultured for 24 h (C, D) and 36 h (A, B) Prior to Fixation. (A) Low power of sub-spherical parasite showing multiple nuclei (N) and abnormally located conoids (C) and groups of rhoptries (R) and micronemes (MN) within the mother cell cytoplasm. Scale bar represents 1 µm. (B) Detail of the enclosed area in (A) showing the conoid and sub-pellicular microtubules (MT) representing the anterior of a daughter. There are normal appearing rhoptries (R) and micronemes (MN) associate with the apex of the daughter but note the absence of an overlying IMC. Scale bar represents 100 nm. (C) Lower showing two parasites that appear to be undergoing endodyogeny in which the developing daughters (D) can be identified. N – nucleus. Scale bar represents 1 µm. (D) Detail of the enclosed area in C showing the presence of the IMC and underlying microtubules (MT) but note the abnormal gaps and overlapping between the plates of the IMC. Scale bar represents 100 nm.