Effect of CD33 and/or CD123 KO in the NPM1-mutated AML cell line OCI-AML3

To reveal which pathways linked to CD33 and CD123 overexpression could be involved in leukemic proliferation, we performed unbiased bulk RNA sequencing comparison between OCI-AML3 wt and CD123 and or CD33 KO clones Overall design: Statistically significant DEGs (adjusted p-value<0.05 and absolute log Fold Change (logFC) > 1.5) were identified for each comparison (OCI-AML3 wt vs -CD33KO, vs -CD123KO and vs -CD33/123KO) using edgeR R studio package.