File S1 - Aspergillus nidulans Cell Wall Composition and Function Change in Response to Hosting Several Aspergillus fumigatus UDP-Galactopyranose Mutase Activity Mutants

Figure S1, Colony morphology and spore colour of Aspergillus nidulans wild type (WT), complemented (WC) strains grown on complete medium at 30°C for 3 d. Both WT and WC strains were grown on same plate. No difference in colony morphology was observed. Arrow indicates respective strains Figure S2, Scanning electron micrographs of Aspergillus nidulans showing: colony (uppercase), and conidiophore (lowercase) phenotype for wild type and SDM strains on complex media. Scale bar is 100 µm for colony images and 10 µm for conidiophore images. WT; wild type (AAE1), WC; complemented with wild type AfugmA (AnugmA::AfugmA), F66A; AnugmA::AfugmA-F66A, H63N; AnugmA::AfugmA-H63N, R182K; AnugmA::AfugmA-R182K, R182A; AnugmA::AfugmA-R182A, R327K; AnugmA::AfugmA-R327K, AnugmAΔ. Figure S3, Strategy for generation of complemented, mutated and GFP-tagged mutated strains in A. nidulans. A. Replacement of A. nidulans ugmA with wild type and mutated A. fumigatus ugmA. B. GFP-tagging of A. nidulans UgmA with wild type and mutated A. fumigatus UgmA. Figure S4, GFP immunolocalization in Aspergillus nidulans using anti-GFP antibody. Wild type complemented (WC) and single residue mutants (H63N, R182A and R327A) have GFP localization comparable to AfUgmA-GFP distribution. Green channel: GFP distribution; Red channel: anti-GFP straining. Bar = 20 µm for all images. Figure S5, Confirmatory PCR for SDM replacement strain. This figure is for replacement of AnugmA by AfugmA-R327A in A. nidulans. In addition to confirmatory PCR, confirmation of all SDM strains was also done by DNA sequencing. Figure S6, Confirmatory PCR for SDM-GFP tagging strains. This figure is for tagging AfugmA-R327A with GFP and expressing in A. nidulans. In addition to Confirmatory PCR, confirmation of all GFP tagged strains was also done by DNA sequencing. Figure S7, Response of wild type, complemented, mutated and deleted strains to Caspofungin. Sensitivity is measured at the innermost clear zone (arrows). WT; wild type, AnugmA::AfugmA, AnugmA::AfugmA-F66A, AnugmA::AfugmA-H63N, AnugmA::AfugmA-R182K, AnugmA::AfugmA-R182A, AnugmA::AfugmA-R327K, AnugmA::AfugmA-R327A, AnugmAΔ. Table S1, Strains and primers used in this study. (DOC)