RNA-sequencing explores the transcriptional profile of Wild Type and DYRK1B transgenic hearts

Here, we investigates the effects of dual-specificity tyrosine-regulated kinase 1B (DYRK1B) on mitochondrial bioenergetics, cardiac hypertrophy, and heart failure.We engineered DYRK1B transgenic and the effects of DYRK1B and its downstream mediators were subsequently elucidated using RNA-seq analysis. In this dataset, we inquire the transcriptional profile of WT and DYRK1B transgenic hearts at 3-day and 8-week old. A total of twelve samples was analyzed. The 3-day old WT (n = 3) and DYRK1B-Tg (n = 3) hearts, 8-week old WT (n = 3) and DYRK1B-Tg ( n = 3) were included in final RNA-sequencing analysis. After genotyping, the hearts were collected and immediately frozen in liquid nitrogen for further analysis. Total RNA was extracted using TRIzol Reagent, reverse-transcribed, and amplified according to the standard procedures. Sequencing libraries were constructed using the BGISEQ-500 platform (BGI, Wuhan, China).