Impact of miR-143-3p and miR-145-5p overexpression on human M238P melanoma cells

To identify putative novel specific targets of miR-143-3p and miR-145-5p, we overexpressed these miRNAs in BRAFV600E mutant human M238P melanoma cells using synthetic mimics or a synthetic “negative” control mimic (miR-Neg). RNA samples were harvested 48 hours post-transfection and 2 independent experiments were carried out. 2 independent experiments performed in a one color design, corresponding to 3 conditions (miR-Neg, miR-143-3p and miR-145-5p) for a total of 6 samples.