Human Cell Landscape at the Single-Cell Level

We used single-cell RNA sequencing to determine cell type composition of all major human organs and construct a basic scheme for the human cell landscape (HCL). We reveal a single-cell hierarchy for many tissues that has not been well characterized previously. We present a ‘‘single-cell HCL analysis’’ pipeline that accurately defines human cell types; and exemplify its utility in stem cell biology. Finally, we perform single-cell comparative analysis for human and mouse cell atlas to reveal the conserved genetic networks in the mammalian system.