Slicer activity of Arabidopsis AGO1 in siRNA biogenesis and miRNA target decay. Slicer activity of Arabidopsis AGO1 in siRNA biogenesis and miRNA target decay

Aim of study The objective of these studies was to analyze the implication of slicer activity of AGO1 in the following molecular processes: • loading of siRNAs and miRNAs • stability of siRNAs and miRNAs • formation of RDR6/SGS3-dependent secondary siRNAs • decay of mRNAs targeted by miRNAs The studies use point mutants defective in slicer activity and analyses total and AGO1-bound small RNA populations as well as mRNA populations in these mutants by next-generation sequencing.