Table 1 in Phestilla subodiosus sp. nov. (Nudibranchia, Trinchesiidae), a corallivorous pest species in the aquarium trade

Table 1. Primers used for PCR and sequencing of Phestilla subodiosus sp. nov.
LCO 14905 ’-GGTCAACAAATCATAAAGATATTGG-3’(Folmer et al. 1994)5 min at 94 °C, 35 × [1min at 94 °C, 30s at 42.5 °C, 1min at 72 °C], 7 min at 72 °CCOIH-25 ’-TAYACYTCRGGATGMCCAAAAATCA-3’(Cella et al. 2016)H3AF5 ’-ATGGCTCGTACCAAGCAGACVGC-3’(Colgan et al. 1998)3min at 94 °C, 35 × [35s at 94 °C, 1min at 50 °C, 1min at 72 °C], 7min at 72 °CH3AR5 ’-ATATCCTTRGGCATRATRGTGAC-3’(Colgan et al. 1998)16S arL5 ’-CGCCTGTTTAACAAAAACAT-3’(Palumbi et al. 2002)3min at 94 °C, 39 × [30s at 94 °C, 30s at 50-55 °C, 1min at 72 °C], 5min at 72 °C16S R5 ’-CCGRTYTGAACTCAGCTCACG-3’(Puslednik and Serb 2008)28SC15 ’-ACCCGCTGAATTTAAGCAT-3’(Dayrat et al. 2001)5min at 94 °C, 35 × [1min at 94 °C, 30s at 45 °C, 1min at 72 °C], 7min at 72 °C28SC25 ’-TGAACTCTCTCTTCAAAGTTCTTTTC-3’(Le et al. 1993)