Sequencing Quantitative Analysis of Stroke+PBS and Stroke+rOPN cortex Transcriptomes

A total of 5 were processed for transcriptome sequencing, generating 39.12Gb Clean Data. At least 6.93Gb clean data were generated for each sample with minimum 94.21% of clean data achieved quality score of Q30. Clean reads of each sample were mapped to specified reference genome. Mapping ratio ranged from 95.86% to 96.09%. Prediction of alternative splicing, gene structure optimization analysis and novel gene discovery was processed on top of mapping results, during which 4,282 were discovered and 2,111 novel genes were annotated with a putative function. The expression of genes was quantified and differentially expressed genes were identified based on their expression. These DEGs were further processed for functional annotation and enrichment analysis.