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Wnt/b-catenin signaling regulates sequential fate decisions of murine cortical precursor cells

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In order to compare expression changes upon Wnt/b-catenin signaling inactivation in the forebrain of mouse embryos, we have isolated dorso-medial pallium from E11.5 mutant and control embryos and performed microarray analysis. Dorso-medial pallium from E11.5 control and mutant embryos was dissected and snap-frozen. RNA isolation on single embryo brains was performed with RNAeasy kit with on-column Dnase digestion.

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