Abiotic Treatment to Common Bean Plants Results in an Altered Endophytic Seed Microbiome

Common bean plants (Phaseolus vulgaris L. (var. Redhawk)) were grown in an environmental growth chamber under 3 different growth conditions: control plants with ample water provided, water-withholding plants given 66% less water, and nutrient-addition plants given 1/2X Hoagland solution (nutrient solution) in place of water. Amplicon sequencing of the V4 region of the 16s rRNA gene and the ITS gene of the seed endophytic DNA from each condition was performed using the Illumina MiSeq platform to assess seed bacterial/archaeal and fungal community structure, respectively. Code for analysis is available on GitHub: https://github.com/ShadeLab/BioRxiv_Seed_Microbiome_2020/blob/master/README.md

普通菜豆植物（Phaseolus vulgaris L. (var. Redhawk)）在环境生长室中，于三种不同的生长条件下培育：充足水分供应的控制植物、水分限制植物（水分供应减少66%）以及添加营养素植物（以1/2X Hoagland营养液（营养溶液）替代水）。针对各条件下的种子内生DNA的16s rRNA基因V4区域和ITS基因，分别采用Illumina MiSeq平台进行扩增子测序，以评估种子细菌/古菌和真菌群落结构。分析代码可在GitHub上获取：https://github.com/ShadeLab/BioRxiv_Seed_Microbiome_2020/blob/master/README.md