Homo sapiens Genome sequencing. Homo sapiens

For more than 30 years, scientists have studied many biological processes in HepG2 cells, publishing some 20,865 articles along the way. This cell is particularly abnormal compared with non-cancer human cell lines, as well as compared with the human reference sequence. However, most genomic studies in HepG2 cells have used the human reference sequence, which is a serious impediment in corresponding genetic level researches using HepG2 cell line such as genomic variation, virus-host genetic interaction and the validating targets of siRNAs, CRISPR-Cas9 and other reagents against a specific sequence. Here, we used Next Generation Sequencing (NGS) to define the HepG2 genome, revealing the true extent of genetic abnormalities at nucleotide resolution, which are valuable resources for the continued use of HepG2 cells in biomedical research.