Wnt/b-catenin signaling regulates sequential fate decisions of murine cortical precursor cells. Mus musculus

In order to compare expression changes upon Wnt/b-catenin signaling inactivation in the forebrain of mouse embryos, we have isolated dorso-medial pallium from E11.5 mutant and control embryos and performed microarray analysis. Overall design: Dorso-medial pallium from E11.5 control and mutant embryos was dissected and snap-frozen. RNA isolation on single embryo brains was performed with RNAeasy kit with on-column Dnase digestion.