RNA-sequencing results of five samples across three YMC cycles of both wt and yaf9W89A strains

The goal of the experiments is to study how the absence of Yaf9-H3 acyl reading affected metabolic and global gene transcription. We performed RNA-seq for our wt and yaf9W89A cells across three consecutive YMC cycles. We found that HOC genes maintained expression in the LOC phase and that LOC genes were not properly expressed in the LOC phase. Overall design: 5 time points of YMC samples were collected across three consecutive cycles for both wt and yaf9W89A strains. Samples WT1-WT5 cover one wt YMC cycle. WT6-WT10 and WT11-WT15 are replicates; Samples W89A16-W89A20 cover one yaf9W89A YMC cycle. W89A21-W89A25 and W89A26-W89A30 are replicates. The triplicates were processed with RNA sequencing technology.