A distinct isoform of lymphoid enhancer binding factor 1 (LEF1) epigenetically restricts EBV reactivation to maintain viral latency

As a human tumor virus, EBV is present as a latent infection in its associated malignancies where genetic and epigenetic changes have been shown to impede cellular differentiation and viral reactivation. We reported previously that levels of the Wnt signaling effector, lymphoid enhancer binding factor 1 (LEF1) increased following EBV epithelial infection and an epigenetic reprogramming event was maintained even after loss of the viral genome. Elevated LEF1 levels are also observed in nasopharyngeal carcinoma and Burkitt lymphoma. To determine the role played by LEF1 in the EBV life cycle, we used in silico analysis of EBV type 1 and 2 genomes to identify over 20 Wnt-response elements, which suggests that LEF1 may bind directly to the EBV genome and regulate the viral life cycle. Using CUT&RUN-seq, LEF1 was shown to bind the latent EBV genome at various sites encoding viral lytic products that included the immediate early transactivator BZLF1 and viral primase BSLF1 genes. The LEF1 g..., Immunofluorescence microscopy images used in the manuscript for quantitation, , # Research data

The experimental procedures are to examine the effect of lymphoid enhancer factor 1 on Epstein-Barr virus reactivation. Knockdown and over expression approaches were used. Two cell types were used (EBV+NOK, epithelial cells) and Akata BX-1 (Burrito's lymphoma cell line).knockdown or over expression analysis were use to evaluate EBV reactivation using BZLF1 and E-AD viral lytic proteins as markers. In addition, LEF1 effects on differentiation were also investigated.

## Description of the data and file structure

The files are raw immunofluorescence images used for quantitation in various assays. Images were captured on the Axis Observer Apothem microscope. The files are raw images used in the companion manuscript and for quantitation.  The images are located in 1 main directory [IF compilation] that contains two subfolders [Images in Paper] and [Images used for quantitation]. These subfolders are subsequently divided into 5 folders organized by the figure that they re...