Transcriptome of adult hearts with cardiomyocyte-specific deficiency of Aars2

We generated cardiomyocyte-specific Aars2 knockout mice using Myh6-Cre, and observed progressive cardiac function decline starting at postnatal day (P) 28, with severe chamber dilation, and phenotypes reminance of mitochondrial cardiomyopathy. All homozygous mutant mice died before reaching P70. We sought to identify the transcriptome changes by Aars2-deficiency at P14 and P21 in adult, prior to the onset of the cardiomyopathy. Overall design: We cross Aars2-Flox mice with Myh6-Cre to generate cardiomyocyte-specific deletion of Aars2. At P14 or P21, heart apex from mutant mice, heterozygous and cre-negative controls were dissected, snap frozen and processed for RNA isolation. Genotype of individual embryos were confirmed by PCR from DNA isolated from tail tips. RNA were processed for rRNA-depleted library construction and RNA-sequencing.