Study design and mortality of mice challenged via the IN route with 2x105 PFU of MPXV.

aBoHV-4 vaccines were administered at T = 0 days via the IP route in a total volume of 0.3 ml at 1x107 PFU. MVA was administered at a dose of 2x107 PFU in a total volume of 0.1 ml injected IM. Veh indicates a DMEM vehicle.bBooster vaccinations were administered as for a.cMice were challenged via the IN route with 2x105 PFU of MPXV. Virus was introduced in a total volume of 25 #x03BC;l to one nare.dRTC, relative to challenge; MTD, mean time to death; SEM, standard error of the mean.eOne mouse was removed due to mortality during blood collection.Study design and mortality of mice challenged via the IN route with 2x105 PFU of MPXV.