Nucleosome position mapping by micrococcal nuclease analysis of S. cerevisiae anchor-away Sth1, Swi2 and/of TBP cells in raffinose and galactose-rich media in rapamycin or DMSO

We analyed the nucleosome positions by using 2 concentrations of micrococcal nuclease on yeast strains that were grown in raffinose or galactose containing media (synthetic complete). Strains contained FRB-tags to Sth1, Swi2, Sth1 & Swi2 or Sth1 and TBP, where TBP was tagged on one of two alleles in a diploid strain. Cells were treated for 60 min either with 7.5 uM rapamycin or the same volume of DMSO. Overall design: Mnase-seq of 4 strains grown in galactose-rich media after treatment with rapamyin or DMSO. Mnase-seq of 1 strain grown in raffinose-rich media after treatment with DMSO.