Mechanomics RT-DC Set 04 - MCF10A-PIK3CA

Entry is a part of Mechanomics collection (10.6084/m9.figshare.c.5399826) that contains materials supporting findings presented in the following manuscript:Urbanska, M., Ge, Y. et al.,De novo identification of universal cell mechanics gene signaturesBiorXiv: https://doi.org/10.1101/2021.04.26.441418; first published: 27th April 2021, last updated: 4th Nov 2024eLife: https://doi.org/10.7554/eLife.87930; first published: 23rd March 2023, last updated: 27th Dec 2024This dataset contains mechanical measurement of MCF10A cell lines with single-allele PIK3CA mutation E545K in exon 9 (ex9) or H1024R in exon 20 (ex20) (Juvin et al., 2013, PLoS One). These cell lines were previously generated by homologous recombination by Horizon Discovery LTD, UK and were kindly provided, together with an isogenic wild type (wt) control, by L.R. Stephens.Lines:• MCF10A wild type (wt)• MCF10A PIK3CA mutation E545K in exon 9 (ex9)*• MCF10A H1024R in exon 20 (ex20).*not used in the final version of the manuscript Measurements were performed using real-time deformability cytometry (RT-DC)._rtdc.zip - contains data acquired during the measurments in '*.rtdc' format_tsv.zip - contains extended data in a '*.tsv' format_glmm.zip - contains result summaries of statistical analysis comparing Young's moduli of the different cell types using generalised linear mixed effects modelsJuvin, V. et al. Signaling via Class IA Phosphoinositide 3-Kinases (PI3K) in Human, Breast-Derived Cell Lines. PLoS One 8, (2013). https://doi.org/10.1371/journal.pone.0075045