Genomic and Transcriptomic Analysis of Chlorotic and Wild-Type Phenotypes in F1 Progeny of an Actinidia chinensis Cross

This study investigates the genetic and transcriptomic basis of chlorosis in the F1 progeny of an Actinidia chinensis cross. In this F1 generation, 25% of the plants display a chlorotic phenotype, while the remaining 75% exhibit a wild-type (wt) phenotype. Two sequencing approaches were performed to differentiate between chlorotic and wt plants: (1) DNA-seq of pooled leaf samples from chlorotic and WT individuals, and (2) RNA-seq of different leaf tissues to examine gene expression differences associated with the chlorotic phenotype.