Enhanced Spacer Length between Mannose Mimicking Shikimoyl and Quinoyl Headgroups and Hydrophobic Region of Cationic Amphiphile Increases Efficiency of Dendritic Cell Based DNA Vaccination: A Structure–Activity Investigation

In the field of dendritic cell based
genetic immunization, previously
we showed that liposomes of cationic amphiphiles containing mannose-mimicking
shikimoyl headgroup are promising DNA vaccine carriers for dendritic
cell (DC) transfection. The present structure–activity study
reports on the influence of spacer length (between mannose-mimicking
headgroups and quaternary nitrogen centers) in modulating the DC-transfection
efficiencies. Further, we report on the anti-melanoma immune response
inducing properties of the promising cationic amphiphiles in syngeneic
C57BL/6J mice under prophylactic settings.