Additional file 1: Figure S1. of Enterococcus hirae biofilm formation on hospital material surfaces and effect of new biocides

In vitro effect of LH IDROXI FAST and LH ENZYCLEAN SPRAY on mature biofilm of S. aureus ATCC 6538. Top; the untreated and treated biofilms were analyzed for the biomass production, after 48 h of incubation at 37 °C on polystyrene surface, through Cristal Violet staining method. The results were expressed as average of OD595 values of three experiments (mean value ± SD). Symbol represents result statistically significant (p ˂ 0.05). Down; representative images of the in vitro mature biofilms at 37 °C on polystyrene surface untreated (a) and treated with LH IDROXI FAST (b) and LH ENZYCLEAN SPRAY (c). Biofilms were cultured for 48 h, stained with live/dead reagents, and visualized with the optical microscope fluorescence. Sessile population in biofilms stained in red (propidium iodide) expresses a compromised membrane integrity (damaged), whereas green stained bacteria (SYTO 9) remained viable. Both biocides reduced significantly S. aureus ATCC 6538 biomasses even exerting a killing effect. Original magnification ×1000. (ZIP 114 kb)