RNA-seq and Ribo-seq of WT and METTL5 KO mECS.
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=a96d4731fbf5aca0743b5f2f1ce2fb1e
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资源简介：
Covalent chemical modifications of cellular RNAs directly impact all biological processes. However, our mechanistic understanding of the enzymes catalysing these modifications, their substrates and biological functions remains vague. Here, we undertook a systematic screen to uncover new RNA methyltransferases. We demonstrate that the methyltransferase-like 5 (METTL5) protein catalyses m6A in 18S rRNA at position A1832. We report that absence of Mettl5 in mouse embryonic stem cells (mESCs) results in a changes gene expression, decrease in translation rate, spontaneous loss of pluripotency and compromised differentiation potential. Mice lacking METTL5 recapitulate symptoms of patients with METTL5 mutations, thereby providing a new mouse disease model. Overall, our work  highlights the importance of m6A in rRNA in stemness, differentiation, development and diseases.
提供机构：
Institute of Functional Epigenetics, Helmholtz Zentrum M�nchen, Deutsches Forschungszentrum fuer Gesundheit und Umwelt (GmbH)
创建时间：
2022-02-20
