Negative regulation of lymphoid specification by the transcription factor TAL1

Lymphoid primed multipotent progenitors are a subset of MPPs that are undergoing specification to the lymphoid and myeloid cell fates. The transcription factors Lyl1 and E2A are both essential for the development LMPPs and can form a heterodimeric complex. We examined the consequences of E2A- or Lyl1-deficiency on development of LMPPs and on their gene expression program in comparison with wild-type LMPPs Examination of RNA present in Rag2-GFP+ and Rag2-GFP- HSC and LMPP isolated from control and VaviCre Tal1floxed/floxed mice.