Additional file 2 of CRISPRi enables isoform-specific loss-of-function screens and identification of gastric cancer-specific isoform dependencies
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https://springernature.figshare.com/articles/dataset/Additional_file_2_of_CRISPRi_enables_isoform-specific_loss-of-function_screens_and_identification_of_gastric_cancer-specific_isoform_dependencies/13647263/1
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Additional file 2: Table S1. Transcript specific RNA-Seq (using the Salomon algorithm) in 4 gastric cancer cell lines. Table S2. Spacer sequences of sgRNAs used in pooled library. Table S3. Gastric cancer specific altered and un-altered transcripts. Table S4. Results from CRISPRi screen. Data was generated using the MAGeCK algorithm. Table S5. Phospho-proteomic mass spectrometry in YCC3 cells following CRISPRi mediated suppression of CIT expression. Table S6. RNA-Seq in YCC3 cells following CRISPRi mediated suppression of CIT expression. Table S7. Primers used in this study for qPCR and generation of sgRNAs.
提供机构：
Mandoli, Amit; Qamra, Aditi; Taotao, Sheng; Chaturvedi, Richa; Tuano, Natasha; Daly, Roger J.; Huang, Kie Kyon; Davies, Rebecca; Itman, Catherine; Tan, Patrick; Powell, David; Liu, Ling; Rosenbluh, Joseph; Hu, Changyuan
创建时间：
2021-01-27
