Maize transcriptomic responses to Pantoea stewartii vascular infection in B73 and pan1 (R2) lines (experiment B).

To gain insight into the genetic and molecular mechanisms that underlie the enhanced resistance of pan1 lines to vascular spread of P. stewartii, we used RNA-seq to compare the transcriptomes of B73 and pan1 lines one day after mock or bacterial inoculation of leaf veins, being this the first analysis of maize transcriptional responses to P. stewartii infection. Overall design: 16 samples were analyzed (2 genotypes x 2 treatments x 4 replicates). Genotyopes: B73 and pan1-Mu/pan1-Mu (R2). Treatments: P. stewartii-GFP infection and Mock inoculation (serving as wounding control). Four biological replicates were analyzed.