Microprism-based two-photon imaging of the mouse inferior colliculus reveals novel organizational principles of the auditory midbrain

To navigate real-world listening conditions, the auditory system relies on the integration of multiple sources of information. However, to avoid inappropriate cross-talk between inputs, highly connected neural systems need to strike a balance between integration and segregation. Here, we develop a novel approach to examine how repeated neurochemical modules in the mouse inferior colliculus lateral cortex (LC) allow controlled integration of its multimodal inputs. The LC had been impossible to study via imaging because it is buried in a sulcus. Therefore, we coupled two-photon microscopy with the use of a microprism to reveal the first-ever sagittal views of the LC to examine neuronal responses with respect to its neurochemical motifs under anesthetized and awake conditions. This approach revealed marked differences in the acoustic response properties of LC and neighboring nonlemniscal portions of the inferior colliculus. In addition, we observed that the module and matrix cellular motif..., Animal subjects:
Male mice of 8-12 weeks of age were used. Males were used as our preliminary data suggest male/female differences in DC organization (Ibrahim, Shinagawa, Xiao, Asilador, & Llano, 2022) and will be published separately. GAD67-GFP (GAD1GFP) knock-in mice (developed and shared with permission from Dr. Yuchio Yanagawa at Gunma University and obtained from Dr. Douglas Oliver at the University of Connecticut), where GFP is exclusively expressed in GABAergic cells (Tamamaki et al., 2003), were used to visualize the GABAergic cells and LC modules. To simultaneously monitor calcium signals and visualize the GFP+ cells, Tg(Thy1-jRGECO1a)GP8.20Dkim/J mice (Jackson Laboratory, Stock# 030525) were crossed with GAD67-GFP knock-in mice to generate GAD67-GFPxThy1-jRGECO1a hybrid mice. The mice were housed and bred at the animal research facility of The Beckman Institute for Advanced Science and Technology. The animal care facilities are approved by the Association for Assessment an..., , # Microprism-based two-photon imaging of the mouse inferior colliculus reveals novel organizational principles of the auditory midbrain

[https://doi.org/10.5061/dryad.v41ns1s70](https://doi.org/10.5061/dryad.v41ns1s70)

## Description of the data and file structure

The data were collected after the 2P imaging of the craniotomy window of the mouse inferior colliculus either directly from the top surface or from the lateral surface using a microprism. After extracting the cellular responses, different parameters were calculated such as best tone frequency (BTF), best modulation frequency (BMF), spectral modulation index (SMI), and temporal modulation index (TMI). The basics of these calculations were discussed in the methods section.

### Files and variables

#### File: Figure_4-figure_supplement_1-data_source_1.xlsx

* **Description:** 

These data are for 8 animals as shown in figure 4. Each sheet of the excel file is for one animal as shown by the name of the sheet. Each sheet shows ...,