Supplementary Material for: CXCR1 Regulates Pulmonary Anti-<b><i>Pseudomonas</i></b> Host Defense

<i>Pseudomonas aeruginosa</i> is a key opportunistic pathogen causing disease in cystic fibrosis (CF) and other lung diseases such as chronic obstructive pulmonary disease (COPD). However, the pulmonary host defense mechanisms regulating anti-<i>P. aeruginosa</i> immunity remain incompletely understood. Here we demonstrate, by studying an airway <i>P. aeruginosa</i> infection model, in vivo bioluminescence imaging, neutrophil effector responses and human airway samples, that the chemokine receptor CXCR1 regulates pulmonary host defense against <i>P. aeruginosa</i>. Mechanistically, CXCR1 regulates anti-<i>Pseudomonas</i> neutrophil responses through modulation of reactive oxygen species and interference with Toll-like receptor 5 expression. These studies define CXCR1 as a novel, noncanonical chemokine receptor that regulates pulmonary anti-<i>Pseudomonas</i> host defense with broad implications for CF, COPD and other infectious lung diseases.铜绿假单胞菌（Pseudomonas aeruginosa）是引发囊性纤维化（CF）及慢性阻塞性肺疾病（COPD）等肺部疾病的关键机会致病菌。然而，调控抗铜绿假单胞菌免疫的肺部宿主防御机制仍未完全阐明。本研究通过气道铜绿假单胞菌感染模型、活体生物发光成像、中性粒细胞效应应答分析及人类气道样本研究，证实趋化因子受体CXCR1可调控肺部抗铜绿假单胞菌的宿主防御功能。机制层面，CXCR1可通过调节活性氧生成及干扰Toll样受体5（Toll-like receptor 5）的表达，调控抗铜绿假单胞菌的中性粒细胞应答。本研究明确CXCR1是一类新型非经典趋化因子受体，可调控肺部抗铜绿假单胞菌的宿主防御，对囊性纤维化、慢性阻塞性肺疾病及其他感染性肺部疾病的研究具有广泛参考价值。