Degradome sequencing of urine-derived ADO2-iPSCs

To identify potential miRNA–mediated targets associated with osteopetrosis at the genome-wide level, two small RNA libraries and a degradome library were constructed from the ADO2-iPSCs and normal control human iPSCs (NC-iPSCs) for deep sequencing. Overall design: Degradome sequencing (DS) was used to validate cleaved targets of the identified miRNAs between the ADO2-iPSCs and NC-iPSCs libraries.