Ebraccatus_Priority_Effects

The EPE was a collection of short term studies in which the eggs of hourglass treefrogs were inoculated with bacteria in different orders in order to test for the presence of priority effects in tadpole bacteria community development. The first experiment was an inoculation experiment with frog embryos using two bacterial isolates. To complement our tadpole experiment, a follow-up experiment was conducted in which broth containing wells within a well-plate were similarly inoculated with bacteria under two nutrient concentrations (Full LB broth or a 1:20 dilution LB broth). We then conducted a third test in which embryos were inoculated first with a single bacterium and then with a synthetic community of bacteria.The sequences included are 16S rRNA sequences of the V4 variable region.

本研究集收录了一系列短期研究，旨在通过将玻璃树蛙的卵接种于不同菌属的细菌，以检验在蝌蚪细菌群落发育过程中是否存在优先效应。首项实验为青蛙胚胎的接种实验，使用两种细菌纯培养物进行。为补充蝌蚪实验，进行了一项后续实验，在该实验中，含有微孔的微量滴定板内的培养基同样接种了细菌，并置于两种营养浓度（全量LB培养基或1:20稀释的LB培养基）下。随后，我们进行了第三项测试，其中胚胎首先接种单一细菌，然后接种由细菌组成的合成群落。所包含的序列为V4可变区的16S rRNA序列。